WebSTEN buffer (detailed below) is a basic IP and wash buffer. For increased stringency, also wash in STEN with 0.5M NaCl, 1% NP-40, and 0.1% SDS. The final wash should be mild to prevent salt or detergent carry-over. Incubate with 1 mL washing buffer for 20 min at 4C with shaking. Spin down beads 12,000g x 20 sec and carefully remove WebTransfer the IP-reactions into the bead-containing tubes prepared and incubate the reaction mixtures for 2 h head-over-tail at 4°C. Spin down the beads and carefully remove and retain the supernatant. Wash the beads with 1 ml of 1x IP buffer three times. Add 100 µl of elution buffer to the sedimented beads.
IP Wash 600 - Projecteurs IP à LEDs - Terre de Son
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