Mcherry egfp
WebThe principle of using Cox8-EGFP-mCherry to monitor mitophagy. When cells are expressing Cox8-EGFP-mCherry either by transient transfection with a Cox8 … WebpCMV-mCherry-p62是碧云天研发的在哺乳动物细胞中表达红色mCherry标签的人源p62融合蛋白的质粒。该质粒含有CMV启动子,为卡那霉素抗性,转染后能够在靶细胞中高效表达带有红色荧光蛋白mCherry标签的p62融合蛋白,呈现明亮的红色荧光,可以用于细胞自噬(autophagy)的研究
Mcherry egfp
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Web南模生物. Language: 中文 . English; 日本語; 한국어; 账户设置; 退出 Web14 nov. 2010 · Strain construction growth conditions and image analysis. All plasmids and strains used in this work are detailed in Table 1.GFP cloning was performed by ligation …
WebLenti-CMV-CD9-EGFP,即Lentivirus expressing CD9-EGFP fusion protein,是碧云天自行研发的一种可以在大多数哺乳动物细胞(包括原代细胞和干细胞)中通过CMV启动子表达 … WebMonitoring autophagic flux is important for the analysis of autophagy. Tandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on different pH stability of EGFP and mRFP fluorescent proteins. However, it has been reported that there is still weak fluo …
WebThe Afp::mCherry transgene is based on and recapitulates reporter expression of a previously described Afp::GFP strain. However, we note that perdurance of mCherry … WebFigure 1. Detection of a palette of fluorescent proteins using the Attune™ NxT Acoustic Focusing Cytometer. 293FT cells or U2OS cells were transfected or transduced with plasmid or viral constructs expressing different fluorescent proteins. Samples were acquired on the Attune™ NxT cytometer at a flow rate of 100 μL/min using 405 nm, 488 nm ...
WebLenti-mCherry-EGFP-LC3B (C3002)和Lenti-EGFP-LC3B (C3003)适合用于一些腺病毒较难感染或者需要筛选稳定细胞株的情况;Ad-mCherry-GFP-LC3B (C3011)和AdPlus …
WebLenti-mCherry-EGFP-LC3B (C3002)和Lenti-EGFP-LC3B (C3003)适合用于一些腺病毒较难感染或者需要筛选稳定细胞株的情况;Ad-mCherry-GFP-LC3B (C3011)和AdPlus-mCherry-GFP-LC3B (C3012)由于表达起始时间短,适合用于能被腺病毒感染并且希望快速获得实验结果的情况,也可以在表达E1的HEK293A、HEK293等适当细胞中扩增,便于后续 ... miss state women\u0027s basketball recruitingWebpCMV-GFP-LC3B是碧云天自行研发的在哺乳动物细胞中表达GFP标签的人源LC3B 融合蛋白的质粒。该质粒含有CMV启动子,为卡那霉素抗性,转染细胞后能够在靶细胞中高效表达绿色荧光蛋白GFP标签的LC3B的融合蛋白,呈现明亮的绿色荧光,可以用于细胞自噬(autophagy)的研究。 miss state womens bask scoresWebMonitoring autophagic flux is important for the analysis of autophagy. Tandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux … miss state women\\u0027s basketball scheduleWebViral Service: Retrograde AAV. As part of our Viral Service, Addgene is producing ready-to-use viral vector preparations in a retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced with the rAAV2-retro helper plasmid (Addgene #81070). This serotype permits robust retrograde ... miss state will rogersWeb18 mrt. 2009 · The red fluorescent protein mCherry is of considerable interest for fluorescence fluctuation spectroscopy (FFS), because the wide separation in color … miss state women\\u0027s basketball rosterWeb14 aug. 2024 · The CAT-R reporter consists of two coding sequences for the fluorescent proteins mCherry and eukaryotic green fluorescent protein (eGFP) linked with a self … miss. state women\u0027s basketball scheduleWebTandem GFP-mCherry-LC3B expression indicates elevated autophagic flux in EGFRvIII + (K) BS153 and (L) DKMG cells during hypoxia (24 h O 2 0.02%). Scale bars: 2 μm. Autophagy inhibition through CQ addition (5 µg/ml) abrogates the survival advantage of EGFRvIII + (M) BS153 and (N) DKMG cells as assessed by crystal violet staining after … miss state women\\u0027s basketball recruiting