Phospho-akt1/2/3 ser473 cellular kit
WebFeb 3, 2024 · Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 phosphorylates many, perhaps hundreds, of downstream cellular targets in the cytosol and nucleus. AKT1 is well-known for phosphorylatin … WebIn the case of AKT1/2, immunofluorescence is reduced following the augmentation of NP-427 concentrations (Figure 8A–D). Similar to the anterior cases, the Western blot showed a decrease in phospho-Thr308-Akt, phospho-Ser473-Akt, and total AKT at 24 h in cells treated with NP-427 at 0.5 and 0.75 mg/mL ( p < 0.05) ( Figure 8 E).
Phospho-akt1/2/3 ser473 cellular kit
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WebThe protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents Product specifications Designed with signal performance in mind, the kit offers a tailored standard curve ideally suited to the study of Gi coupled GPCRs. Introduction to product validation WebThe phospho-AKT1 (Ser473) kit is designed for robust quantification of AKT1 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), Akt is an oncogene …
Web#IR172-668 / IR172-668,Phospho-AKT (Ser473) antibody. Products ... and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. ... Subsequent phosphorylation of both threonine residue 308 and serine ... WebMMP1 Antibody SKU: A...
LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells. WebSep 15, 2024 · The protein concentration of the supernatants was measured by BCS TM protein assay kit according to manufacturer’s ... The knockout of STK24 expression did …
WebAKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation.
WebMMP1 Antibody SKU: A... citi macy\\u0027s loginWebThis assay kit contains antibodies which recognize the phospho-Ser473 epitope, and a distal epitope, common to Akt 1, Akt 2 and Akt 3. The proteins detected by this kit correspond to … citi makemytrip offerWebProduct Note This antibody is raised against human AKT1 phosphorylated at Ser473. Based on sequence homology, it is predicted to react with AKT2 and AKT3 when phosphorylated at the corresponding residues - AKT2 Ser474 and AKT3 Ser472. Predict Reactivity Zebrafish, Sheep, Bovine, Cat, Chicken, Rhesus Monkey (>80% identity) PROPERTIES TARGET diastole of the purkinje fibers is what waveWebPhospho-AKT1 (Ser473) Antibody (700392) in ICC/IF Immunofluorescent analysis of AKT (pS473) was done on 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. diastole wortherkunftWebAKT, also known as protein kinase B, is an important regulator of numerous cellular processes including cell survival, glucose metabolism, transcription, and apoptosis. AKT … citi mailing address credit cardsWebPhospho-AKT1/2/3 (Ser473) cellular kit HTRF® Kit Diabetes and metabolic disorders A portfolio to tackle in-depth diabetes and metabolic disorders studies Explore Phospho-AKT1/2/3 (Ser473) control lysate HTRF® Control lysate Phospho-AKT1/2/3 (Thr308) cellular kit HTRF® Kit Phospho-AKT1/2/3 (Thr308) control lysate HTRF® Control lysate Guides diastole is another name for muscleWeb(Ser473) Chemiluminescent Sandwich ELISA Kit detects endogenous levels of phospho-Akt1 (Ser473) in human, rat and mouse cells. Please visit www.cellsignal.com for a complete listing of recommended companion products. #7134C PathScan® Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit Species Cross-Reactivity: H, M, R … diastole wealth management inc