Smad3 western blot
WebbSMAD3 antibody (pAb) tested by Western blot. Whole cell extract of A549 cells (30 µg) probed with SMAD3 antibody (1:500). Background. This SMAD3 (mothers against … WebbSmad3 is phosphorylated by TGF-b, heterodimerizes with Smad4 and enters the nucleus. The heterodimer binds within the promoter of plasminogen activator inhibitor-1 (PAI-1) and induces its expression. Sequence Synthetic peptide located within the following region: FTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAIT Physical form
Smad3 western blot
Did you know?
WebbD-G, HK-2 cells were transfected with an siRNA against EZH2 or a negative control siRNA (si-NC) for 48 h before being exposed to H/R. D-F, Western blot analysis for the protein … Webb3 sep. 2024 · western blot knockout validation; human; 1:1000; loading ...; fig 1c Gori I, George R, Purkiss A, Strohbuecker S, Randall R, Ogrodowicz R, et al . Mutations in SKI in …
WebbIt was further verified in relative cell lines by real-time quantitative polymerase chain reaction and Western blotting (WB). Adenocarcinoma cell lines were transfected with si-NC and si- AHNAK2 by lipofectamine 3000 and treated with or without TGF-β 1, and cell migration and invasion were detected by wound-healing and transwell assays. WebbSMAD3 Antibody has been tested for use in ELISA and by western blot. This antibody is suitable in immunohistochemistry. Specific conditions for reactivity should be optimized …
WebbA human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain. It is a receptor regulated … Webb7 apr. 2024 · i Western blot analysis of Flag-NP, p-Smad3, Ripk3, p-MLKL and MLKL expression in the kidneys of 16-week-old db/m and db/db mice with or without overexpressing SARS-CoV-2 N protein.
Webb5 feb. 2024 · Total protein samples were extracted from the cells and equal amounts of total protein (20 μg) were subjected to Western blotting analysis. The levels of indicated proteins were assessed by a standard immuno-blotting technique with a specific primary antibody. Actin indicates equal amounts of protein loading into each lane (left panel).
Webbcells and Th17 cells. The specificity of the O72-670 mAb was confirmed by Western blot and immunohistochemistry using unconjugated antibody. The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. fish eating birds listWebbWestern blot analysis of SMAD3 (Fig. a) was performed by loading 20 µg of HeLa Control (lane 1), HeLa SMAD3 knockout (lane 2) whole cell extracts. SMAD3 was detected at 53 … canada battery kelownaWebbWestern blot assay The purified cells treated with MENK and untreated cells were lysed in cell disruption buffer (mirVana TMPARIS RNA and Native Protein Purification Kit; Invitrogen Life Technology, CA). Protein samples (40 mg) were subjected to electrophoretic analysis through 10% SDS-PAGE43 and subsequently transferred fish eating cartoonWebbReceptor-regulated Smads (R-Smads), Smad2 and Smad3, are directly phosphorylated and activated by ALK5. Phosphorylation occurs at C-terminal SS X S motifs and promotes the … fish eating creek campground flWebb30 dec. 2016 · Smad3 Antibody (38-Q) is an IgG2a κ mouse monoclonal Smad3 antibody suitable for the detection of Smad3 of mouse, rat and human origin by WB, IP, IF, IHC (P) … canada bathroom vanitiesWebb28 juli 2005 · Normal fibroblasts (NF) and keloid fibroblasts (KF) were co-cultured in serum-free medium with normal keratinocytes (NK) or keloid keratinocytes (KK) for 5 days, after … fisheating creek campgroundWebb30 sep. 1997 · (Lower) Western blot analysis of Smad3 protein levels in the transfected cells demonstrate equivalent expression of the wild-type and mutant Smad3 proteins. … fisheating creek campground florida